Article
Antisense inhibition of hMLH1 is not sufficient for loss of DNA mismatch repair function in the HCT116+chromosome 3 cell line.
Clinical cancer research : an official journal of the American Association for Cancer Research - 1 Oct 2000
Chauhan D P, Yang Q, Carethers J M, Marra G, Chang C L, Chamberlain S M, Boland C R
Abstract excerpt
We have reported that transfer of chromosome 3 (Chr3) containing a single wild-type copy of the hMLH1 gene into HCT116 colon cancer cells, a cell line deficient in DNA mismatch repair (MMR) activity attributable to inactivating hMLH1 mutations, corrects all of the aspects of the MMR repair-deficient phenotype. We inhibited the expression of the wild-type hMLH1 gene using antisense RNA in HCT116+Chr3 cells to...
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