Article
Effect of hMSH6 cDNA expression on the phenotype of mismatch repair-deficient colon cancer cell line HCT15.
Carcinogenesis - 1 Mar 1999
Lettieri T, Marra G, Aquilina G, Bignami M, Crompton N E, Palombo F, Jiricny J
Abstract excerpt
Mismatch recognition in human cells is mediated primarily by a heterodimer of hMSH2 and hMSH6. Cells mutated in both alleles of the hMSH6 gene are deficient in the correction of base/base mispairs and insertion/deletion loops of one nucleotide and thus exhibit a strong mutator phenotype, evidenced by elevated mutation rates and microsatellite instability, as well as by tolerance to methylating agents. The...
Topics
- Base Pair Mismatch
- Base Sequence
- Colonic Neoplasms
- DNA Primers
- DNA Repair
- DNA, Complementary
- DNA-Binding Proteins
- G2 Phase
- Guanine
- Humans
- Methylnitronitrosoguanidine
- Methylnitrosourea
- Microsatellite Repeats
- Mutation
- Phenotype
- Transfection
- Tumor Cells, Cultured