Article
HSV-1 infected cell proteins influence tetracycline-regulated transgene expression.
The journal of gene medicine - 1 Jan 2000
Herrlinger U, Pechan P A, Jacobs A H, Woiciechowski C, Rainov N G, Fraefel C, Paulus W, Reeves S A
Abstract excerpt
BACKGROUND: This study investigates elements of herpes simplex virus type 1 (HSV-1) which influence transgene expression in tetracycline-regulated expression systems. METHODS: Different HSV-1 mutants were used to infect Vero cells that had been transfected with plasmids containing the luciferase gene under the control of tet-off or tet-on tetracycline-regulation systems. RESULTS: The baseline level of luciferase...
Topics
- Animals
- Chlorocebus aethiops
- Gene Expression
- Gene Transfer Techniques
- Genes, Immediate-Early
- Herpesvirus 1, Human
- Immediate-Early Proteins
- Luciferases
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Tetracycline
- Transcription, Genetic
- Transfection