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Article

Detection of kinases that phosphorylate 14-3-3 binding sites of Raf-1 using in situ gel kinase assay.

Biological & pharmaceutical bulletin - 1 Oct 2000

Kinuya M, Takishima K, Mamiya G

Abstract excerpt

Raf-1 is a serine/threonine protein kinase that plays a critical role in mitogenic signal transduction. Raf-1 activation requires 14-3-3 binding to Raf-1 as an essential step. This binding is regulated through phosphorylation of Ser259 and Ser621 of Raf-1, each constituting part of the consensus motif for the binding of Raf-1 to 14-3-3. However, Raf-1 kinase kinase(s) that phosphorylates these sites remains...

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Source

Identifiers

PMID: 11041243
DOI: 10.1248/bpb.23.1158

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EGF

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Detection of kinases that phosphorylate 14-3-3 binding sites of Raf-1 using in situ gel kinase assay.PMID 11041243Read ↗

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