Article
Rapid methods for population-scale analysis for gene polymorphisms: the ACE gene as an example.
British heart journal - 1 Apr 1995
O'Dell S D, Humphries S E, Day I N
Abstract excerpt
OBJECTIVE: To obtain rapid, high throughput genotyping of the angiotensin converting enzyme (ACE) gene intron 16 insertion/deletion polymorphism. METHODS: DNA was obtained from whole blood samples by a simple liquid phase methanol extraction procedure. The ACE gene was amplified by the polymerase chain reaction (PCR) using two oligonucleotide primers (ACE1 and ACE3) outside the insertion sequence and one primer...
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