Article
Frequency of minisatellite repeat number changes at the MS205 locus in human sperm before and after cancer chemotherapy.
Environmental and molecular mutagenesis - 1 Jan 2000
Zheng N, Monckton D G, Wilson G, Hagemeister F, Chakraborty R, Connor T H, Siciliano M J, Meistrich M L
Abstract excerpt
To determine whether the measurement of repeat number mutations at a minisatellite locus could detect human germline mutations induced by chemotherapy, we performed a longitudinal study of the mutation frequencies in sperm from 10 patients treated for Hodgkin's disease. Polymerase chain reaction on small pools of DNA equivalent to 100 sperm and Southern blotting were used to screen at least 7900 sperm in each...
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