Article
Evaluation of ABO and Lewis genotypes using primer extension preamplification.
Forensic science international - 11 Sept 2000
Itoh Y, Kobayashi R
Abstract excerpt
There are some difficulties with blood typing from ABO variant bloodstains and Lewis negative samples using serologic methods. In these samples, DNA analysis should be employed simultaneously to avoid errors in typing. Primer extension preamplification (PEP) produces copies of template DNA. The minimum quantity to examine nucleotide substitutions of ABO and Lewis genotypes by PCR ranged from 1 to 3 ng DNA. The...
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