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Article

A simple method for the determination of individual rate constants for substrate hydrolysis by serine proteases.

Protein science : a publication of the Protein Society - 1 Aug 2000

Ayala Y M, Di Cera E

Abstract excerpt

A simple method is presented for the determination of individual rate constants for substrate hydrolysis by serine proteases and other enzymes with similar catalytic mechanism. The method does not require solvent perturbation like viscosity changes, or solvent isotope effects, that often compromise nonspecifically the activity of substrate and enzyme. The rates of substrate diffusion into the active site (k1),...

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Identifiers

PMID: 10975580
DOI: 10.1110/ps.9.8.1589
PMCID: PMC2144722

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Source receipt: pubmed, retrieved 7 Sept 2026.

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