Article
Development of a semi-quantitative assay to detect full-length CYP2C19 RNA.
BioTechniques - 1 Aug 2000
Wang X Y, Levy R H, Ho R J
Abstract excerpt
Cytochrome P450 2C19 (CYP2C19) is the enzyme responsible for the metabolism of a number of drugs, including anticonvulsants and antidepressants. We have developed a semi-quantitative competitive RT-PCR assay to estimate the degree of expression of the full-length CYP2C19 message. This assay used a known quantity of internally deleted CYP2C19 RNA to quantitate the RT-PCR products of the CYP2C19 transcript in the...
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