Article
Telomerase-dependent repeat divergence at the 3' ends of yeast telomeres.
Nucleic acids research - 15 Jul 2000
Förstemann K, Höss M, Lingner J
Abstract excerpt
Yeast telomeres consist of approximately 300 nt of degenerate repeats with the consensus sequence G(2-3)(TG)(1-6). We developed a method for the amplification of a genetically marked telomere by PCR, allowing precise length and sequence determination of the G-rich strand including the 3' terminus. We examined wild-type cells, telomerase RNA deficient cells and a strain deleted for YKU70, which encodes for a...
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