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Article

Expression, purification, and characterization of cysteine-free mouse P-glycoprotein.

Archives of biochemistry and biophysics - 1 Jan 2006

Tombline Gregory, Urbatsch Ina L, Virk Navneet, Muharemagic Alma, White Lori Bartholomew, Senior Alan E

Abstract excerpt

Cysteine-free mouse MDR3 P-glycoprotein (Pgp) was constructed by mutagenesis of the nine natural Cys to Ala. The Cys-free protein was expressed in Pichia pastoris and purified. Yield, purity, ATPase activity, K(m)(MgATP), and stimulation of ATPase by verapamil, were similar to wild-type mouse Ppg. Mouse Cys-free Pgp was superior in yield and stability to Cys-free human MDR1 Pgp. Mutants Y1040A and Y1040C were...

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Identifiers

PMID: 16343415
DOI: 10.1016/j.abb.2005.11.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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