Article
Dissection of the functional differences between sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 and 2 isoforms and characterization of Darier disease (SERCA2) mutants by steady-state and transient kinetic analyses.
The Journal of biological chemistry - 28 Nov 2003
Dode Leonard, Andersen Jens Peter, Leslie Natalie, Dhitavat Jittima, Vilsen Bente, Hovnanian Alain
Abstract excerpt
Steady-state and rapid kinetic studies were conducted to functionally characterize the overall and partial reactions of the Ca2+ transport cycle mediated by the human sarco(endo)plasmic reticulum Ca2+-ATPase 2 (SERCA2) isoforms, SERCA2a and SERCA2b, and 10 Darier disease (DD) mutants upon heterologous expression in HEK-293 cells. SERCA2b displayed a 10-fold decrease in the rate of Ca2+ dissociation from E1Ca2...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Amino Acid Sequence
- Animals
- Blotting, Western
- Calcium
- Calcium-Transporting ATPases
- Catalysis
- Cell Line
- DNA, Complementary