Article
Comparative genetic and physiological studies of the MAP kinase Mpk1p from Kluyveromyces lactis and Saccharomyces cerevisiae.
Journal of molecular biology - 21 Jul 2000
Kirchrath L, Lorberg A, Schmitz H P, Gengenbacher U, Heinisch J J
Abstract excerpt
MAP kinases are essential components of signal transduction pathways in yeasts and higher eukaryotes. Here, we report on the isolation of the gene encoding the MAP kinase KlMpk1p by complementation of the respective Saccharomyces cerevisiae deletion mutant with a genomic library from Kluyveromyce...
Topics
- Amino Acid Sequence
- Benzenesulfonates
- Caffeine
- Cloning, Molecular
- Fungal Proteins
- Genetic Complementation Test
- Kluyveromyces
- Microbial Sensitivity Tests
- Mitogen-Activated Protein Kinases
- Molecular Sequence Data
- Osmolar Concentration
- Phenotype
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Alignment
- Sequence Deletion
- Sodium Dodecyl Sulfate
- Two-Hybrid System Techniques