Article
A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans.
Journal of clinical microbiology - 1 Apr 2000
Stinear T, Davies J K, Jenkin G A, Portaels F, Ross B C, Oppedisano F, Purcell M, Hayman J A, Johnson P D
Abstract excerpt
Two high-copy-number insertion sequences, IS2404 and IS2606, were recently identified in Mycobacterium ulcerans and were shown by Southern hybridization to possess restriction fragment length polymorphism between strains from different geographic origins. We have designed a simple genotyping method that captures these differences by PCR amplification of the region between adjacent copies of IS2404 and IS2606. We...
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