Article
Development and Application of Two Multiplex Real-Time PCR Assays for the Detection of <i>Mycobacterium ulcerans</i> in Clinical and Environmental Samples
26 May 2007
Abstract excerpt
Mycobacterium ulcerans is a slow-growing environmental bacterium that causes a severe skin disease known as Buruli ulcer. PCR has become a reliable and rapid method for the diagnosis of M. ulcerans infection in humans and has been used for the detection of M. ulcerans in the environment. This paper describes the development of a TaqMan assay targeting IS2404 multiplexed with an internal positive control to...
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