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Article

Thermal stability effects of removing the type-2 copper ligand His306 at the interface of nitrite reductase subunits.

European biophysics journal : EBJ - 1 Sept 2007

Stirpe Andrea, Sportelli Luigi, Wijma Hein, Verbeet Martin Ph, Guzzi Rita

Abstract excerpt

Nitrite reductase (NiR) is a highly stable trimeric protein, which denatures via an intermediate, N(3)<--(k)-->U(3)--(k)-->F (N-native, U-unfolded and F-final). To understand the role of interfacial residues on protein stability, a type-2 copper site ligand, His306, has been mutated to an alanine. The characterization of the native state of the mutated protein highlights that this mutation prevents copper ions...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 17701241
DOI: 10.1007/s00249-007-0151-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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