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Article

Site-directed mutagenesis of rat thioltransferase: effects of essential cysteine residues for the protection against oxidative stress.

Journal of biochemical and molecular toxicology - 1 Jan 2000

Terada Tomoyuki, Okamoto Kei-ichi, Nishikawa Jun-ichi, Miura Takeshi, Nishinaka Toru, Nishihara Tsutomu

Abstract excerpt

A cDNA of rat liver thioltransferase was cloned and then expressed using pMAL-c expression vector in Escherichia coli. Recombinant rat liver thioltransferase was expressed as a fusion protein with maltose-binding protein and then purified by amylose resin column chromatography to be homogeneity on 12.5% SDS-polyacrylamide gel electrophoretic analysis. The expressed proteins were shown as two bands at around 53...

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PMID: 20175144
DOI: 10.1002/jbt.20312

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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