Article
A missense mutation in the nuclear gene coding for the mitochondrial aspartyl-tRNA synthetase suppresses a mitochondrial tRNA(Asp) mutation.
Nucleic acids research - 1 Apr 2000
Chiang C S, Liaw G J
Abstract excerpt
The nuclear suppressor allele NSM3 in strain FF1210-6C/170-E22 (E22), which suppresses a mutation of the yeast mitochondrial tRNA(Asp)gene in Saccharomyces cerevisiae, was cloned and identified. To isolate the NSM3 allele, a genomic DNA library using the vector YEp13 was constructed from strain E22. Nine YEp13 recombinant plasmids were isolated and shown to suppress the mutation in the mitochondrial...
Topics
- Alleles
- Amino Acid Sequence
- Aspartate-tRNA Ligase
- Cell Nucleus
- Cloning, Molecular
- DNA, Fungal
- DNA, Mitochondrial
- Escherichia coli
- Gene Amplification
- Genes, Fungal
- Mitochondria
- Molecular Sequence Data
- Mutation, Missense
- Plasmids
- RNA, Transfer, Asp
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid
- Suppression, Genetic