Article
Establishment and properties of a growth factor-dependent, perpetual neural stem cell line from the human CNS.
Experimental neurology - 1 Jan 2000
Villa A, Snyder E Y, Vescovi A, Martínez-Serrano A
Abstract excerpt
The ready availability of unlimited quantities of neural stem cells derived from the human brain holds great interest for basic and applied neuroscience, including therapeutic cell replacement and gene transfer following transplantation. We report here the combination of epigenetic and genetic procedures for perpetuating human neural stem cell lines. Thus we tested various culture conditions and genes for those...
Topics
- Blotting, Southern
- Brain
- Cell Culture Techniques
- Cell Differentiation
- Cell Division
- Cell Line, Transformed
- Central Nervous System
- Epidermal Growth Factor
- Fibroblast Growth Factor 2
- Gene Expression Regulation, Viral
- Genes, gag
- Genes, myc
- Genetic Therapy