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Article

Substrate range and genetic analysis of Acinetobacter vanillate demethylase.

Journal of bacteriology - 1 Mar 2000

Morawski B, Segura A, Ornston L N

Abstract excerpt

An Acinetobacter sp. genetic screen was used to probe structure-function relationships in vanillate demethylase, a two-component monooxygenase. Mutants with null, leaky, and heat-sensitive phenotypes were isolated. Missense mutations tended to be clustered in specific regions, most of which make known contributions to catalytic activity. The vanillate analogs m-anisate, m-toluate, and...

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Identifiers

PMID: 10671462
DOI: 10.1128/JB.182.5.1383-1389.2000
PMCID: PMC94427

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