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Article

Mutagenesis of soluble methane monooxygenase.

Methods in enzymology - 1 Jan 2011

Smith Thomas J, Murrell J Colin

Abstract excerpt

The hydroxylase component of soluble methane monooxygenase (sMMO), which is the site of oxidation of methane and many adventitious substrates of commercial and environmental interest, has proved challenging to manipulate genetically because of difficulties with obtaining functional expression in Escherichia coli. Here, we describe methods that allow site-directed mutagenesis of the hydroxylase-encoding genes and...

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PMID: 21419919
DOI: 10.1016/B978-0-12-386905-0.00009-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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