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Article

Application of the effects of ionic strength reducing agents in the purification and crystallization of chitinase A.

Acta crystallographica. Section D, Biological crystallography - 1 Oct 2002

Papanikolau Yannis, Petratos Kyriacos

Abstract excerpt

The effects of ionic strength reducing agents may find a large number of applications. Based on these effects, we have redesigned the purification scheme of Chitinase A (ChiA) from Serratia marcescen. This scheme led to reproducibly crystallizable enzyme in both salting-in and salting-out conditions, which are presented here. Herein, we demonstrate some experimental applications of the ionic strength reducing...

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Source

Identifiers

PMID: 12351868
DOI: 10.1107/s0907444902014191

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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