Article
Simple PCR detection of haptoglobin gene deletion in anhaptoglobinemic patients with antihaptoglobin antibody that causes anaphylactic transfusion reactions.
Blood - 15 Feb 2000
Koda Y, Watanabe Y, Soejima M, Shimada E, Nishimura M, Morishita K, Moriya S, Mitsunaga S, Tadokoro K, Kimura H
Abstract excerpt
Two anhaptoglobinemic patients showing anaphylactic transfusion reactions by antihaptoglobin antibody were found. Southern blot analysis indicated that 2 patients were homozygous for the deleted allele of the haptoglobin gene (Hp(del)) as reported previously. We have identified the junction region of the deletion from genomic DNA of 1 patient using cassette-mediated polymerase chain reaction (PCR). Then, the...
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