Article
Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study.
Microbiology (Reading, England) - 1 Jan 2000
Hirose Isao, Sano Kazuyoshi, Shioda Izumi, Kumano Miyuki, Nakamura Kouji, Yamane Kunio
Abstract excerpt
To analyse the proteome of Bacillus subtilis extracellular proteins, extracellular protein samples were prepared from culture media (minimal medium containing 0.4% glucose) of parental B. subtilis 168, a secA-temperature sensitive mutant and an ffh conditional mutant, and examined by two-dimensional gel electrophoresis. Approximately 100 to 110 spots were visualized in a gel of B. subtilis 168 extracellular...
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