Article
The reconstructed epidermis with melanocytes: a new tool to study pigmentation and photoprotection.
Cellular and molecular biology (Noisy-le-Grand, France) - 1 Nov 1999
Cario-André M, Bessou S, Gontier E, Maresca V, Picardo M, Taïeb A
Abstract excerpt
The reconstruction of the epidermal melanin unit ex vivo has been achieved during the last decade, using the combination of previous cell culture techniques. The system reviewed is basically a modification of the Pruniéras model, using the air-liquid interface to grow differentiated keratinocytes, with the addition of 5% melanocytes in the seeding suspension, as well as the use of a more adapted culture medium....
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