Article
Faithful in vitro transcription by fission yeast RNA polymerase III reveals unique alpha-amanitin sensitivity.
Gene expression - 1 Jan 1999
Rödicker F, Ossenbühl F, Michels D, Benecke B J
Abstract excerpt
Transcription with fission yeast (Schizosaccharomyces pombe) RNA polymerase III (pol III) was studied in two different in vitro systems. Reactions performed with isolated nuclei gave rise to 5S and pre-tRNA molecules. Because the alpha-amanitin sensitivity of that reaction clearly differed from what has been observed with pol III enzymes of other eukaryotes, a cell-free S. pombe transcription extract was...
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