Article
Mutant Caenorhabditis elegans RNA polymerase II with a 20,000-fold reduced sensitivity to alpha-amanitin.
Genetics - 1 Dec 1990
Rogalski T M, Golomb M, Riddle D L
Abstract excerpt
A doubly mutant ama-1(m118m526) gene results in an RNA polymerase (Rpo) II that is unusually resistant to alpha-amanitin. Rpo II activity in isolated Caenorhabditis elegans cell nuclei is inhibited 50% by alpha-amanitin at a concentration of 150 micrograms/ml, making this enzyme 150 times more resistant to the toxin than Rpo II from the singly mutant allele, ama-1(m118), 20,000 times more resistant than the...
Topics
- Amanitins
- Animals
- Blotting, Southern
- Caenorhabditis
- Drug Resistance
- Genes, Dominant
- Mutation
- Phenotype
- RNA Polymerase II
- Temperature