Article
Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach.
Molecular & general genetics : MGG - 1 Dec 1999
Entian K D, Schuster T, Hegemann J H, Becher D, Feldmann H, Güldener U, Götz R, Hansen M, Hollenberg C P, Jansen G, Kramer W, Klein S, Kötter P, Kricke J, Launhardt H, Mannhaupt G, Maierl A, Meyer P, Mewes W, Munder T, Niedenthal R K, Ramezani Rad M, Röhmer A, Römer A, Hinnen A
Abstract excerpt
In a systematic approach to the study of Saccharomyces cerevisiae genes of unknown function, 150 deletion mutants were constructed (1 double, 149 single mutants) and phenotypically analysed. Twenty percent of all genes examined were essential. The viable deletion mutants were subjected to 20 different test systems, ranging from high throughput to highly specific test systems. Phenotypes were obtained for...
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