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Article

A reliable PCR amplification method for microdissected tumor cells obtained from paraffin-embedded tissue.

Genetic analysis : biomolecular engineering - 15 Dec 1999

Akalu A, Reichardt J K

Abstract excerpt

We report a reliable method for PCR (polymerase chain reaction) amplification of genomic DNA from PET. This method uses DNA extraction with the QIAquick kit and amplification with AmpliTaq Gold. Amplification of up to 959 bp from PET was achieved with this combination which exceeds the current reported upper limit of 800 bp. In summary, the gradual activation of the AmpliTaq Gold during thermal cycling allows...

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Identifiers

PMID: 10609759
DOI: 10.1016/s1050-3862(99)00050-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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