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Article

Genotyping of DNA samples isolated from formalin-fixed paraffin-embedded tissues using preamplification.

The Journal of molecular diagnostics : JMD - 1 Nov 2010

Baak-Pablo Renee, Dezentje Vincent, Guchelaar Henk-Jan, van der Straaten Tahar

Abstract excerpt

DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue is often fragmented and cross-linked and is therefore difficult to genotype. To enable this source of DNA for genotyping analysis using Taqman probes, we tested whether enrichment of the target genes would increase the amount of available DNA. For enrichment of the target genes, we used preamplification by means of diluted Taqman assays. To...

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Identifiers

PMID: 20847277
DOI: 10.2353/jmoldx.2010.100047
PMCID: PMC2963916

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