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Article

Efficient DNA extraction for HPV genotyping in formalin-fixed, paraffin-embedded tissues.

The Journal of molecular diagnostics : JMD - 1 Jul 2011

Steinau Martin, Patel Sonya S, Unger Elizabeth R

Abstract excerpt

DNA from archived FFPE can be used for papillomavirus genotyping, but potential problems include paraffin as a physical barrier, DNA cross-linking, and PCR inhibitors. To address these complications, we combined a commercially available DNA isolation kit (Qiagen DNeasy) with a heat treatment and evaluated the resulting DNA with regards to HPV typing. DNA was extracted from 10-μm sections from 150 FFPE cancer...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 21704270
DOI: 10.1016/j.jmoldx.2011.03.007
PMCID: PMC3123789

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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