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Article

Intrabody construction and expression III: Engineering hyperstable <i>V<sub>H</sub></i> domains

1 Jan 1999

Abstract excerpt

The folding of immunoglobulin domains requires the formation of a conserved structural disulfide. Therefore, as a general rule, they cannot be functionally expressed in the reducing environment of the cellular cytoplasm. We have previously reported that stability engineering can lead to the cytoplasmic expression of functional immunoglobulin V(L) domains. Here we apply rational stability engineering by consensus...

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Identifiers

PMID: 10595527
DOI: 10.1110/ps.8.11.2245

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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