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Article

Intrabody construction and expression. II. A synthetic catalytic Fv fragment.

Journal of molecular biology - 3 Sept 1999

Ohage E C, Wirtz P, Barnikow J, Steipe B

Abstract excerpt

In general, proteins with structural disulfides cannot be expressed in the reducing environment of the cellular cytoplasm. To overcome this folding problem, we have previously engineered stabilizing mutations, predicted from a consensus sequence analysis, into isolated immunoglobulin VL domains. Here we show that such domains can be used as a framework in the construction of a functional heterodimeric Fv...

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Source

Identifiers

PMID: 10518948
DOI: 10.1006/jmbi.1999.3020

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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