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Characterization of the P140K, PVP(138-140)MLK, and G156A O6-methylguanine-DNA methyltransferase mutants: implications for drug resistance gene therapy.

Human gene therapy - 20 Nov 1999

Davis B M, Roth J C, Liu L, Xu-Welliver M, Pegg A E, Gerson S L

Abstract excerpt

The G156A O6-alkylguanine-DNA alkyltransferase (AGT) mutant protein, encoded by the G156A O6-methylguanine-DNA methyltransferase gene (MGMT), is resistant to O6-benzylguanine (BG) inactivation and, after transduction into hematopoietic progenitors, transmits remarkable resistance to BG and BCNU. As a result, a clinical trial, in which the MGMT gene is transduced into CD34+ cells of patients with cancer, has been...

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Source

Identifiers

PMID: 10584923
DOI: 10.1089/10430349950016500

Genes

MGMT
AGT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Characterization of the P140K, PVP(138-140)MLK, and G156A O6-methylguanine-DNA methyltransferase mutants: implications for drug resistance gene therapy.PMID 10584923Read ↗

Retroviral-mediated expression of the P140A, but not P140A/G156A, mutant form of O6-methylguanine DNA methyltransferase protects hematopoietic cells against O6-benzylguanine sensitization to chloroethylnitrosourea treatment.Semantic proximity Read ↗

The P140K mutant of human O(6)-methylguanine-DNA-methyltransferase (MGMT) confers resistance in vitro and in vivo to temozolomide in combination with the novel MGMT inactivator O(6)-(4-bromothenyl)guanine.Semantic proximity Read ↗

Differential competitive resistance to methylating versus chloroethylating agents among five O6-alkylguanine DNA alkyltransferases in human hematopoietic cells.Semantic proximity Read ↗

Protection of hematopoietic cells against combined O6-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O6-methylguanine DNA methyltransferase.Semantic proximity Read ↗

Interaction of mammalian O(6)-alkylguanine-DNA alkyltransferases with O(6)-benzylguanine.Semantic proximity Read ↗

Protection of CHO cells by mutant forms of O6-alkylguanine-DNA alkyltransferase from killing by 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) plus O6-benzylguanine or O6-benzyl-8-oxoguanine.Semantic proximity Read ↗

Lentiviral Transduction of P140K MGMT into Human CD34+ Hematopoietic Progenitors at Low Multiplicity of Infection Confers Significant Resistance to BG/BCNU and Allows Selection in VitroSemantic proximity Read ↗

Tumor-associated mutations in O⁶ -methylguanine DNA-methyltransferase (MGMT) reduce DNA repair functionality.Semantic proximity Read ↗

Myeloablation is not required to select and maintain expression of the drug-resistance gene, mutant MGMT, in primary and secondary recipients.Semantic proximity Read ↗

Mutations in human O6-alkylguanine-DNA alkyltransferase imparting resistance to O6-benzylguanine.Semantic proximity Read ↗

Clinical Relevance of <i>MGMT</i> in the Treatment of CancerSemantic proximity Read ↗

Creation of human alkyltransferases resistant to O6-benzylguanine.Semantic proximity Read ↗

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