Retroviral-mediated expression of the P140A, but not P140A/G156A, mutant form of O6-methylguanine DNA methyltransferase protects hematopoietic cells against O6-benzylguanine sensitization to chloroethylnitrosourea treatment.

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Article

Retroviral-mediated expression of the P140A, but not P140A/G156A, mutant form of O6-methylguanine DNA methyltransferase protects hematopoietic cells against O6-benzylguanine sensitization to chloroethylnitrosourea treatment.

The Journal of pharmacology and experimental therapeutics - 1 Sept 1999

Maze R, Kurpad C, Pegg A E, Erickson L C, Williams D A

Abstract excerpt

O(6)-Benzylguanine (6-BG) inactivates mammalian O(6)-methylguanine DNA methyltransferase (MGMT), an important DNA repair protein that protects cells against chloroethylnitrosourea (CENU) cytotoxicity. 6-BG is being tested as an approach to treat CENU-resistant tumors that overexpress endogenous MGMT. However, in addition to restoring CENU tumor cell sensitivity, 6-BG also increases the cytotoxic effects of CENUs...

Topics

Animals
Antineoplastic Agents
Carcinoma
Ethylnitrosourea
Female
Genetic Vectors
Guanine
Hematopoietic Stem Cells
Humans
Leukemia L1210

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Melanoma, Experimental

Mice

Mice, Inbred C57BL

Mutation

O(6)-Methylguanine-DNA Methyltransferase

Retroviridae

Tumor Cells, Cultured

Source

Open in PubMed

Identifiers

PMID: 10454526

Genes

MGMT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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DNA repair methyltransferase (Mgmt) knockout mice are sensitive to the lethal effects of chemotherapeutic alkylating agents.Semantic proximity Read ↗

Lentiviral Transduction of P140K MGMT into Human CD34+ Hematopoietic Progenitors at Low Multiplicity of Infection Confers Significant Resistance to BG/BCNU and Allows Selection in VitroSemantic proximity Read ↗

Protection and in Vivo Selection of Hematopoietic Stem Cells Using Temozolomide, O6-Benzylguanine, and an Alkyltransferase-Expressing Retroviral VectorSemantic proximity Read ↗

Myeloablation is not required to select and maintain expression of the drug-resistance gene, mutant MGMT, in primary and secondary recipients.Semantic proximity Read ↗

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