Article
Permeabilization of fungal membranes by plant defensins inhibits fungal growth.
Applied and environmental microbiology - 1 Dec 1999
Thevissen K, Terras F R, Broekaert W F
Abstract excerpt
We used an assay based on the uptake of SYTOX Green, an organic compound that fluoresces upon interaction with nucleic acids and penetrates cells with compromised plasma membranes, to investigate membrane permeabilization in fungi. Membrane permeabilization induced by plant defensins in Neurospora crassa was biphasic, depending on the plant defensin dose. At high defensin levels (10 to 40 microM), strong...
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