Article
Enhanced monomer-monomer interactions can suppress the recombination deficiency of the recA142 allele.
Molecular microbiology - 1 Oct 1999
Zaitsev E N, Kowalczykowski S C
Abstract excerpt
The RecA142 protein, in which valine is substituted for isoleucine-225, is defective for genetic recombination in vivo and for DNA strand exchange activity in vitro under conventional growth and reaction conditions respectively. However, we show that mildly acidic conditions restore both the in vitro DNA strand exchange activity and the in vivo function of RecA142 protein, suggesting that recombination function...
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