Article
Specific defects in double-stranded DNA unwinding and homologous pairing of a mutant RecA protein.
FEBS letters - 14 Jul 2000
Kurumizaka H, Aihara H, Ikawa S, Shibata T
Abstract excerpt
The DNA molecules bound to RecA filaments are extended 1.5-fold relative to B-form DNA. This extended DNA structure may be important in the recognition of homology between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). In this study, we show that the K286N mutation specifically impaired the dsDNA unwinding and homologous pairing activities of RecA, without an apparent effect on dsDNA binding itself....
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