Article
Two fatty acid delta9-desaturase genes, ole1 and ole2, from Mortierella alpina complement the yeast ole1 mutation.
Microbiology (Reading, England) - 1 Oct 1999
Wongwathanarat P, Michaelson L V, Carter A T, Lazarus C M, Griffiths G, Stobart A K, Archer D B, MacKenzie D A
Abstract excerpt
Genes encoding two distinct fatty acid delta9-desaturases were isolated from strains of the oleaginous fungus Mortierella alpina. Two genomic sequences, delta9-1 and delta9-2, each containing a single intron, were cloned from strain CBS 528.72 while one cDNA clone, LM9, was isolated from strain CBS 210.32. The delta9-1 gene encoded a protein of 445 aa which shared 99% identity with the LM9 gene product. These...
Topics
- Fatty Acid Desaturases
- Genes, Fungal
- Genetic Complementation Test
- Molecular Sequence Data
- Mortierella
- Mutation
- Saccharomyces cerevisiae
- Stearoyl-CoA Desaturase