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Article

Isolation and characterization of mutations affecting expression of the delta9- fatty acid desaturase gene, OLE1, in Saccharomyces cerevisiae.

FEBS letters - 18 Aug 1997

Fujimori K, Anamnart S, Nakagawa Y, Sugioka S, Ohta D, Oshima Y, Yamada Y, Harashima S

Abstract excerpt

Expression of the delta9- fatty acid desaturase gene, OLE1, of Saccharomyces cerevisiae is negatively regulated transcriptionally and post-transcriptionally by unsaturated fatty acids. In order to isolate mutants exhibiting irregulation of OLE1 expression, we constructed an OLE1p-PHO5 fusion gene as a reporter consisting of the PHO5 gene encoding repressible acid phosphatase (rAPase) under the control of the OLE1...

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PMID: 9280286
DOI: 10.1016/s0014-5793(97)00846-6

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