Article
Sensitivity of shuttle vector plasmid pZ189 DNA to carbon ion beams.
International journal of molecular medicine - 1 Nov 1999
Harada K, Ueda T, Ando M, Sawada S, Yamanaka H, Tamura Y, Watanabe H, Nagaoka S, Nakano T, Mukai C, Ohnishi T
Abstract excerpt
The sensitivity of shuttle vector plasmid pZ189 DNA to carbon ion beams was studied under dry conditions. This plasmid contains the supF gene that can be used in mutagenesis analysis. The survival curve of the plasmid had both a shoulder and exponential parts, and showed a D10 value of about 17 kGy for both Escherichia coli wild-type and DNA repair-deficient mutant (UV-sensitive) strains. Therefore, it was...
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