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Article

Improved assay sensitivity of an engineered secreted Renilla luciferase.

Gene - 3 Sept 1999

Liu J, Escher A

Abstract excerpt

We have previously reported the construction of a functional Renilla luciferase enzyme secreted by mammalian cells when fused to the signal peptide of human interleukin-2. The presence of three predicted cysteine residues in the amino acid sequence of Renilla luciferase suggested that its secreted form could contain oxidized sulfhydryls, which might impair enzyme activity. In this work, four secreted Renilla...

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PMID: 10524246
DOI: 10.1016/s0378-1119(99)00314-5

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