Sequence analysis and expression of the aspartokinase and aspartate semialdehyde dehydrogenase operon from rifamycin SV-producing amycolatopsis mediterranei.

Abstract excerpt

A approximately 4.8 kb KpnI fragment, from the upstream region of the methylmalonyl-CoA mutase gene (mutAB) of rifamycin SV-producing Amycolatopsis mediterranei, was cloned and partially sequenced. Codon preference analysis showed three complete ORFs. ORF2 is internal to ORF1, and encodes a polypeptide corresponding to 172 amino acids, whereas ORF1 encodes a polypeptide of 421 amino acids. They were identified as...

Topics

• Actinobacteria
• Amino Acid Sequence
• Aspartate Kinase
• Aspartate-Semialdehyde Dehydrogenase
• Cloning, Molecular
• DNA, Bacterial
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Gene Expression Regulation, Enzymologic
• Genetic Complementation Test
• Methylmalonyl-CoA Mutase
• Molecular Sequence Data