Article
Does trifluoroethanol affect folding pathways and can it be used as a probe of structure in transition states?
Nature structural biology - 1 Sept 1999
Main E R, Jackson S E
Abstract excerpt
Nonaqueous co-solvents, particularly 2,2,2-trifluoroethanol (TFE), have been used as tools to study protein folding. By analyzing FKBP12, an alpha/beta-protein that folds with two-state kinetics, we have been able to address three key questions concerning the use of TFE. First, does TFE perturb the folding pathway? Second, can the observed changes in the rate of folding and unfolding in TFE be attributed to a...
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