Article
Automated detection of prevalent mutations in BRCA1 and BRCA2 genes, using a fluorogenic PCR allelic discrimination assay.
Genetic testing - 1 Jan 2000
Abbaszadegan M R, Struewing J P, Brown K M, Snider J V, Goodsaid F, Gore-Langton R, Hughes M R
Abstract excerpt
Mutations in the genes BRCA1 and BRCA2 account for 5%-10% of familial early onset breast cancer. Identification of these mutations allows molecular diagnosis for breast cancer susceptibility. A high through-put automated PCR allelic discrimination assay (ADA) was developed to detect the prevalent mutations in these genes. Two allele specific oligonucleotides (ASO) are directly used in the PCR reaction, in both of...
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