Article
Construction of an artificial tandem protein of the c-Myb DNA-binding domain and analysis of its DNA binding specificity.
Biochemical and biophysical research communications - 19 Aug 1999
Oda M, Furukawa K, Sarai A, Nakamura H
Abstract excerpt
An artificial tandem protein was generated using the third repeat of the c-Myb DNA-binding domain, and its DNA binding affinity and specificity were analyzed by a filter binding assay, isothermal titration calorimetry, and surface plasmon resonance. Although this artificial protein had the proper secondary structure, which is similar to the third repeat by itself, it could not bind to the expected base sequences...
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