Article
Fractionation and enrichment of oligodendrocytes from developing human brain.
Journal of neuroscience research - 1 Aug 1999
Grever W E, Zhang S, Ge B, Duncan I D
Abstract excerpt
Enriched cultures of human oligodendrocytes were obtained from fetal brain specimens between 16 and 21 gestational weeks. Brain cells were separated over a Percoll density gradient and collected as two fractions with initial relative densities of approximately 1.035 g/ml and 1.102 g/ml, for fractions 1 and 2, respectively. After separation, 58.3 and 67.7% of the cells in fractions 1 and 2, respectively, were...
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