Article
Rapid quantitation of gene therapy specific CFTR expression using the amplification refractory mutation system.
BioTechniques - 1 Jul 1999
Thorpe P H, Porteous D J
Abstract excerpt
Gene therapy offers the potential of correcting genetic disorders such as cystic fibrosis (CF). By complementing the non-functional endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene with a functional transgene, we anticipate it may alleviate the disease phenotype. All approaches to CF gene therapy rely upon sensitive assays to monitor delivery, expression and maintenance of CFTR vectors....
Topics
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA Primers
- DNA, Complementary
- Fluorometry
- Gene Amplification
- Gene Expression
- Genetic Therapy
- Humans
- Mutation
- RNA, Messenger
- Reverse Transcriptase Polymerase Chain Reaction
- Software
- Transfection
- Transgenes