Article
Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR.
Molecular human reproduction - 1 Dec 2003
Pierce Kenneth E, Rice John E, Sanchez J Aquiles, Wangh Lawrence J
Abstract excerpt
We present a method for rapid and accurate identification of the normal and DeltaF508 alleles of the cystic fibrosis (CF) gene in single human cells that utilizes LATE (linear after the exponential)-PCR, a newly invented form of asymmetric PCR. Detection of the single-stranded amplicon is carried out in real time, using allele-specific molecular beacons. The LATE-PCR method permits controlled abrupt transition...
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