Role of metal ions in the hydrolysis reaction catalyzed by RNase P RNA from Bacillus subtilis.

doi:10.1006/jmbi.1999.2890

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Article

Role of metal ions in the hydrolysis reaction catalyzed by RNase P RNA from Bacillus subtilis.

Journal of molecular biology - 9 Jul 1999

Warnecke J M, Held R, Busch S, Hartmann R K

Abstract excerpt

Precursor tRNA (ptRNA) substrates carrying a single Rp or Sp-phosphorothioate modification at the RNase P cleavage site were used as tools to study the cleavage mechanism of RNase P RNA from Bacillus subtilis. Both the Sp and the Rp-diastereomer reduced the rate of processing at least 10(4)-fold under conditions where the chemical step is essentially rate-limiting. Neither the Rp nor the Sp-phosphorothioate...

Topics

Bacillus subtilis
Base Sequence
Binding Sites
Cadmium
Calcium
Catalysis
Endoribonucleases
Escherichia coli
Escherichia coli Proteins
Hydrogen-Ion Concentration
Hydrolysis
Magnesium
Manganese
Metals
Mutation
RNA Precursors
RNA, Bacterial
RNA, Catalytic

Source

Open in PubMed

Identifiers

PMID: 10390342
DOI: 10.1006/jmbi.1999.2890

Provenance

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